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 X-ray crystallography

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updated Thu. April 18, 2019

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The research team at IIT lead by Dr Pravindra Kumar from Department of Biotechnology used the state of the art technique of X-ray crystallography to look deep into the bacterial metabolic pathways. It unravelled the molecular basis of bacterial inhibition caused by the compound.
The ppGpp molecule interacts with E. coli's RNA polymerase — the cellular machine that produces RNA from genomic DNA — but precisely how this interaction controls gene expression remains a mystery. The new X-ray crystal structures, however, provide clues to this process by showing for the first time ...

The ppGpp molecule interacts with E. coli's RNA polymerase -- the cellular machine that produces RNA from genomic DNA -- but precisely how this interaction controls gene expression remains a mystery. The new X-ray crystal structures, however, provide clues to this process by showing for the first time ...
The researchers squeezed crystals of copper(I) m-carborane-9-thiolate (Cu-S-M9) in a diamond anvil cell. At 12 gigapascals of pressure, a copper-sulfur bond broke and copper(I) got reduced to copper(0), according to measurements with transmission electron microscopy and energy dispersive X-ray ...
Pomona, CA – Cal Poly Pomona has added a new Single Crystal X-Ray Diffractometer (XRD) to its scientific equipment to expand the width of faculty research on campus and to give students experience with high quality research equipment. The XRD, the D8 Venture model made by Bruker, is one of the ...
At DESY's X-ray source PETRA III, scientists have followed the growth of tiny wires of gallium arsenide live. Their observations reveal exact details of the growth process responsible for the evolving shape and crystal structure of the crystalline nanowires. The findings also provide new approaches to ...

At DESY's X-ray source PETRA III, scientists have investigated an intriguing form of self-assembly in liquid crystals: When the liquid crystals are filled into cylindrical nanopores and heated, their molecules form ordered rings as they cool -- a condition that otherwise does not naturally occur in the material.
"The X-ray pulses excite the electrons," explains first author Tavella. "If just about 1.5 per cent of the electrons are excited, the crystal already starts to change its internal organisation, flipping to the graphite state." The observations not only settle the question on how diamond graphitizes when excited with ...
Deerfield Beach, FL -- (SBWIRE) -- 12/08/2017 -- Global X-Ray Crystallography Instruments Market 2017 presents a far reaching and basic investigation of X-Ray Crystallography Instruments business along the edge of the examination of subjective angles that is ready to exhibit key business bits of knowledge to the ...
For this technique, a crystal has to be grown from the protein first. Researchers then take X-ray snapshots from all sides of the crystal that has to be cooled to ultra-low temperatures to reduce damage from the intense radiation. The X-rays produce a characteristic diffraction pattern from which the inner ...
Scientists at DESY have developed novel lenses that enable X-ray microscopy with record resolution in the nanometre regime. Using new ... This high efficiency is achieved with the layered structures that make up the lens and which act like an artificial crystal to diffract X-rays in a controlled way. The high ...
They determine important data about line-shaped defects that largely influence the deformation behavior of crystals. These so-called dislocations impede the production of computer chips. As reported in Physical Review Letters, the scientists combine two X-ray methods with a special type of light ...
The X-rays carries an invaluable source of information when applied to the chemical systems. Various X-ray based techniques are used nowadays in chemistry, for e.g. X-ray absorption spectroscopy, X-ray diffraction (Single crystal and powder) etc. The technique of X-ray diffraction is widely pursued by ...
Using X-ray beams and lasers, researchers studied how a new promising class of solar cell materials, called hybrid perovskites, behaves at the nanoscale level during operation. Their experiments revealed that when voltage is applied, ions migrate within the material, creating regions that are no longer as ...
In addition to the PIC-mapping study, which used a tool known as a photoemission electron microscope (PEEM) at the ALS, separate ALS experiments used a 3-D imaging technology known as X-ray microtomography and another X-ray method known as microdiffraction to further analyze the crystal ...
An X-ray-based technique known as PIC mapping shows the size and orientation of fibers at the back (left), the middle (center), and the tip (right) of the enameloid layer of a parrotfish's biting tooth. The orientation angle of the crystals is color-coded (chart at bottom). The imaging technique revealed a chain ...
Using X-ray crystallography, the researchers uncovered the 3-D molecular structure of the Ds-Px base pair during DNA replication by analyzing ...

Karle, who worked as chief scientist in the X-ray Diffraction Section at the ... the crystallographic community was skeptical about its usefulness ...
Today, the most common structures in drug discovery have been derived using X-ray crystallography, a technique that usually provides a single ...
It involves diffracting X-rays off planes of electron density and using the ... But X-ray diffraction crystallography is the best way we have to image ...
The X-rays were then passed through a crystal that converted them into ... computing technologies, and the challenges of applying X-ray to a ...
... Nanoscale Virus Features from Correlations of Scattered X-rays ... high-resolution molecular structure has been X-ray crystallography, where ...
The X-ray crystallography of molecules involves exposing a lattice of regularly spaced molecules to a beam of X-rays. Interference of the ...
They used the meso-crystallization technique to obtain crystalline samples of the proteins by growing them in a “cubic lipid mesophase” – a medium that allows the protein to move freely without leaving the membrane. They then irradiated the crystals with X-rays with a wavelength of about 1 angstrom, ...
BioCryst Pharmaceuticals logo BioCryst Pharmaceuticals, Inc. (NASDAQ:BCRX) has been given an average rating of "Buy" by the eight research firms that are currently covering the firm.
Here is an image of the X-ray crystal structure of the Axl/Gas6 complex where Gas6 domains are green and Axl domains are magenta.
The purified and crystallized proteins can be analyzed with X-ray crystallography or Nuclear Magnetic Resonance. This allows the structure of every protein encoded by the genome to be determined.
X-ray Diffractometer (XRD) is a laboratory-based technique commonly used for identification of crystalline materials and analysis of unit cell dimensions.
"The virus only encodes three proteins, so we knew this one must be important," said Tao, whose lab analyzes the structure and function of molecules through X-ray crystallography and electron microscopy. "By studying Orsay's replication cycle, we ...
Bernard Swaim, 60, talks to defense attorney Nick Yedinak in his first court appearance Monday. He's charged with first-degree murder for allegedly killing Stephen E. Smith of Cashmere, in a cold case dating back 35 years.
Scientists from Imperial College London have revealed how materials such as gold can help create protein crystals. The team, from the Department of Surgery and Cancer, hope their findings, published in the journal Scientific Reports, could aid the ...
MIPT scientists found a way to transfer membrane proteins embedded in nanodiscs directly to the lipid-based crystallization matrix and grow membrane protein crystals suitable for high resolution X-ray diffraction studies. In the newly proposed approach ...
But Watson and Crick weren't working in a vacuum, as Howard Markel writes for Scientific American. Just 50 miles away, he writes, scientists at King's College, London were studying DNA as well, using X-ray crystallography. "One of them, Rosalind ...
For protein X-ray crystallography, we make a crystal of our protein of interest - many millions of copies of the protein, all slotting together in a highly ordered way.
To this end, I gave a talk at the UK's National Portrait Gallery (NPG) earlier this month (ahead of International Women's Day on 8 March), discussing the lives and work of the five female structural biologists in the NPG collection - all X-ray ...
The X-Ray Crystallography Market report provides in-depth analysis of parent market trends, macro-economic indicators and governing factors along with market attractiveness as per segments.
Then she sent X-rays through that crystal to begin uncovering the ribosome's structure, pinpointing the locations of the hundreds of thousands of atoms that compose it.
John T. Randall's Biophysics Unit at King's College London, where she used X-ray crystallography to investigate the structure of DNA.
The Compact Dynamic Beamstop (CDBS), which won an R&D 100 Award in 2016, allows scientists to extract the maximum possible amount of data from each precious protein crystal by carefully monitoring and optimizing the X-ray dose. It is a breakthrough ...
Lee Wachtstetter, a Floridian honoring her husband's dying wish that she continue cruising after he died in 1997, has lived ever since aboard Crystal Cruises' Crystal Serenity luxury cruise ship. .... While ships typically have excellent medical ...
... His experimental and theoretical studies on the diffraction of light by acoustic waves of ultrasonic and hypersonic frequencies (published 1934-1942), and those on the effects produced by X-rays on infrared vibrations in crystals exposed to ...
In 2015, his lab used X-ray crystallography to establish the structure of an Au133 nanoparticle and found that it contained complex, self-organized patterns that mirrored patterns found in nature.
The researchers managed to characterize the protein using x-ray crystallography, a method that allowed them to determine how the structure of the protein's lipid transfer fold came to be.
The Company focuses on the treatment of rare diseases. The Company uses X-ray crystallography, computer modeling of molecular structures and chemistry techniques to focus on the three-dimensional molecular structure and active site characteristics of ...
Their groundbreaking research focuses on the use of X-ray radiation in fields as varied as protein crystallography, earth science, paleontology, chemistry and physics.
X-Ray Crystallography is the study of crystal structures, when an X-ray beam bombards them. The diffraction pattern is created by the X-ray diffraction technique, produces wavelengths, which are in the same order of magnitude and interatomic distances.
... an X-ray free-electron laser then intersects with a droplet that contains protein crystals. (SLAC National Accelerator Laboratory).
"Cryo-TEM has great potential in drug discovery applications; it is highly complementary to traditional methods, such as x-ray crystallography and nuclear magnetic resonance," said Denis Zeyer, CEO, NovAliX. "For small molecules research, cryo-TEM ...
... led by graduate student Chenjie Zeng, established the structure of Au246, one of the largest and most complex nanoparticles created by scientists to date and the largest gold nanoparticle to have its structure determined by X-ray crystallography.
In the case of DNA, it was X-ray crystallography, Mendelian genetics, biochemistry, molecular biology. All of those things kind of fused together into an exotic cocktail that led to the discovery of the structure of DNA.


 

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X‑ray crystallographs